Tottenham Hotspur have called for tough action against West Ham United fans who were shown on a video clip chanting a "vile" anti-Semitic song.
The video, filmed by a passenger on the London Underground, appears to show West Ham supporters chanting anti-Semitic songs before Sunday's 2-2 draw at White Hart Lane.
The latest clip shows a group of fans standing in the Tube chanting "I've got foreskin, how about you? F******Jew."
West Ham have said any fan behaving inappropriately faces a possible life ban. The latest incident comes days after a racist incident involving Chelsea football fans on a Paris Metro train.
Tottenham, who have historic links to the Jewish community, said they hope those involved are identified and dealt with seriously.
A Spurs spokesman said: "Anti-Semitism in any form is wholly unacceptable and we support all efforts to kick it out of the game.
"We hope that those individuals responsible for this vile behaviour are identified and dealt with in the strongest way possible."
The footage was uploaded to Twitter by Spurs fan @RomanGeezer, who said: "On the train on the way to the game, West Ham fans break into song in Stamford Hill, a Jewish area, scum."
British Transport Police is investigating the latest incident and a spokesman said: "We take these incidents very seriously, and it is clear what happened on the Paris Metro and at St Pancras the following night were not isolated events."
West Ham said they had emailed every supporter who bought a ticket to Sunday's game to remind them they were acting as ambassadors for the club.
A spokesman said: "If any individual is found to have behaved in an inappropriate way, the club's simple, zero-tolerance policy dictates that they will face the strongest possible action, including the option of a life ban from the Boleyn Ground.
"If anyone has any information on such behaviour we would urge them to report it to the club, Kick It Out or the police so it can be investigated thoroughly."
Lord Ouseley, the chairman of anti-discrimination group Kick It Out, condemned the incident allegedly involving West Ham supporters.
He said: "What would have happened if we hadn't seen this footage or it hadn't been reported? We need to give people the confidence to challenge discriminatory and abusive behaviour, and this can only be reinforced by action being taken by the police and within football by the clubs and authorities.
"Anti-Semitism must be tackled with the same vigour as all other forms of discrimination. There is a heightened level of concern within the Jewish community about anti-Semitism with some feeling unable to live their everyday lives without fear of harassment, abuse and violence."
At the weekend, Scotland Yard released images of three men in connection with their investigation into a group of supporters who pushed a black commuter off a train and chanted a racist song in the French capital on Tuesday ahead of Chelsea's Champions League clash with Paris St Germain.
Ouseley praised the approach taken by Chelsea in banning five supporters from Stamford Bridge.
"I think Chelsea have been excellent in the way they approached things," he told Press Association Sport.
"They came right out of the traps and took responsibility for the fans even though they would rather those individuals didn't call themselves Chelsea fans.
"Chelsea have set a new standard in that you have to be responsible first and foremost. You (the club) didn't send these people to go out and behave like that - you don't ask your fans to conduct themselves in public like that.
"What we can't do is say it is nothing to do with us. It requires the whole of football to get more involved and take action and at the present time it doesn't seem as though we have that as a response."
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